Fig. 7

Validation of DEGs of interest identified from RNA-seq experiments by RT-qPCR. Genes tested are related to behavior and developmental disorders, adhesion, and ion channels. a, b Comparison of relative gene expression in cExN NPCs for the UM, IS, and AP by (a) RNA-seq and (b) RT-qPCR, including expression analysis of genes related to ‘behavior and developmental disorders’. c-e Comparison of gene expression between the UM, IS, and AP for the cIN NPCs by (c) RNA-seq and by (d, e) RT-qPCR, both for genes that were (d) differentially expressed only in the cIN NPCs, and (e) for genes that were differentially expressed in both cExN and cIN NPCs. P values *P < 0.05, **P < 0.01, ***P < 0.001 were determined by an unpaired t test and all other pairwise comparisons had a non-signficant P value (P ≥ 0.05). RT-qPCR data shown includes n = 3 or more biological replicates from one clonal line per subject, where samples were generated for each subject by using a second clonal iPSC line that differed from the line used for RNA-seq analysis