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Fig. 4 | Molecular Autism

Fig. 4

From: Increased Ca2+ signaling in NRXN1α+/− neurons derived from ASD induced pluripotent stem cells

Fig. 4

Spontaneous Ca2+ transient properties were significantly altered in the day 100 NRXN1α +/− neurons. a, b The representative images of control (a) and patient (b) cells loaded with Fluo-4 AM, displaying dense network of neurons in day 100 neuronal cultures. c, d Representative raster plots showed the spontaneous Ca2+ transient activity of the cells from control (c) and patient (d) cultures. e, f Representative waterfall traces of spontaneous Ca2+ transients in 250 cells over 300 s of recording. g The percentage of spontaneous Ca2+ transients remained unchanged between the two groups. h The frequency of spontaneous Ca2+ transients was significantly increased in the NRXN1α+/− deletion patient cells. i The duration of calcium transients was significantly longer in the NRXN1α+/− deletion patient cells. j The amplitude of spontaneous Ca2+ transient was significantly increased in the NRXN1α+/ deletion patient cells. Control n = 74 recordings/26 coverslips from 6 control iPSC lines (patient n = 47 recordings/21 coverslips) from 6 NRXN1α +/− iPSC lines (Additional file 1: Table S10). All data summary are mean ± SEM. Statistical significance (**p < 0.01, ***p < 0.001, ****p < 0.0001) was evaluated using the Mann-Whitney U test. Bar = 100 μm in a, b. Representative images are from control line 4CCX1 and patient ND1C1

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