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Fig. 6 | Molecular Autism

Fig. 6

From: The role of ubiquitin ligase E3A in polarized contact guidance and rescue strategies in UBE3A-deficient hippocampal neurons

Fig. 6

a Representative confocal images of WT and AS HNs, transfected with α-actinin-RFP vector and cultured on GRs; the underlying GR pattern is reported as inset, scale bars = 50 μm. Cells have been counterstained with DAPI (blue). b Analysis of α-actinin directionality: representative directionality peaked histograms (on the left) are reported for WT and AS neurons, showing the fitted Gaussian curves and the calculation of the α-actinin directionality values (δ), normalized to the GR direction (set at 0°). In the graph (on the right), the mean value of α-actinin directionality for HNs cultured on flat standard surfaces (WT and AS pooled together) is also reported, as symbol (n = 2 for Flat, reported as mean ± SD); #P < 0.05 WT vs. AS, Student t test. c Representative confocal images of WT and AS HNs transfected with α-actinin-RFP and zoomed on soma compartments; scale bars = 10 μm. d, e Analysis of α-actinin signal periodicity in neuronal soma compartment by Plot profile and FFT analysis: d representative examples of Plot profile graphs obtained from the same ROI on the correspondent GRs (left) and α-actinin (right) images, for a WT neuron; e the FFT amount of the α-actinin signal is quantified at the GR frequency pick, normalized to the relative RFP intensity, and reported as α-actinin periodicity (in a.u.); the mean value for HNs cultured on flat standard surfaces (WT and AS together) is reported as symbol. n = 3 for GRs; n = 2 for Flat (here reported mean ± SD). #P ≤ 0.05 WT vs. AS, Student t test

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