Fig. 2

Morphological analysis of WT, AS, WT+UBE3A, and AS+UBE3A neurons (DIV4) cultured on GRs. a–c Axonal morphological analysis: axon alignment to GR pattern direction (°) (a), straightness (b), and mean length (μm) (c); a **P < 0.01 AS vs. WT and vs. WT+UBE3A, Bonferroni’s test; b *P < 0.05 WT vs. AS, Bonferroni’s selected test. d–f Dendritic network morphological parameters: dendrite alignment to GRs (°) (d), straightness (e), and total dendritic tree length (μm) (f); in f, ^#P = 0.05 WT vs. AS, Student’ t test. g–i Axonal secondary branching analysis: axon secondary branches alignment to GRs (°) (g), the percentage of neurons with branches in the axon (over the total number of neurons analyzed) (h), and the amount of axonal branches/neuron (in μm) (i); h ^#P < 0.05 WT vs. AS, Student’ t test; i *P < 0.05 AS vs. WT Bonferroni’s selected test. N ≥ 4, at least 10 HNs for samples