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Table 1. Optimized design and schedule for our visual transitive inference touchscreen task in mice

From: Mutations in neuroligin-3 in male mice impact behavioral flexibility but not relational memory in a touchscreen test of visual transitive inference

StageDesignTrials/sessionPerformance criterion
Training stage 1:
Stimulus exposure
Exposure to all stimulus pairs to facilitate flexibility: 1 session of each stimulus pair in order: A+B; B+C; C+D; D+E52 trials/session; Total 4 sessionsNone
Training stage 2: Premise stimulus pair learningLearning each pairwise discrimination to criterion in order: A+B; B+C; C+D; D+E52 trials/session; until criterion≥ 41/52 correct (79% accuracy) in a session, plus one of the two previous or successive sessions at ≥ 34/52 correct (≥ 65% accuracy)
Training stage 3:
Serial integration
All stimulus pairs presented in serial order: A+ B; B+ C; C+ D; D+ E then sequence repeated with 13 trials of each pair presented within a session52 trials/session; until criterion≥ 37/52 correct (≥ 70% accuracy) in a session or max. 15 sessions (780 trials)
Training stage 4: Pseudorandom integrationAll stimulus pairs (A+B; B+C; C+D-; D+E) presented in pseudorandom order with 13 trials of each pair presented within a session52 trials/session; until criterion≥ 37/52 correct (≥ 70% accuracy) in a session or max. 15 sessions (780 trials)
Transitive Inference Test20 unrewarded trials each of novel pairs AE and BD; 8 trials each of the learned pairs (A+B, B+C, C+D, D+E); all pairs presented in pseudorandom order within a session72 trials/session; 1 sessionNone