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Fig. 2 | Molecular Autism

Fig. 2

From: CYFIP1 overexpression increases fear response in mice but does not affect social or repetitive behavioral phenotypes

Fig. 2

CYFIP1-overexpressing mice have no deficits in social communication or repetitive behaviors. a Ultrasonic vocalizations (USVs) provoked by pup/dam separation were assessed in pups at postnatal days 3, 5, 7, 9, and 12 for 5 min at each time point. There was a significant increase in the number of vocalizations at day 3 in Tg line #2 compared to their WT littermates (WT = 176.6 ± 20.8, Tg #2 = 269.3 ± 24.2). b CYFIP1 mice were tested for social deficits using the three-chamber social test. Both WT and Tg mice spent more time interacting with another mouse when given the choice between a stimulus mouse and an empty cup (social sniff: Line 1: WT = 110.4 ± 7.4, Tg #1 = 97.719 ± 5.5. Non-social sniff: Line 1 WT: 76.2 ± 5.1, Tg #1 = 63.2 ± 4.3. Social Sniff: Line 2: WT = 101.8 ± 6.9, Tg #2112.6 ± 8.3. Non-social sniff, WT = 49.3 ± 4.0, Tg #2, 57.4 ± 5.6). c, d Repetitive behaviors were assessed by scoring time spent grooming (c) and digging (d) over a 10-min period. No excessive grooming or digging was noted in CYFIP1 Tg mice (Grooming: Line 1: WT = 85.85 ± 11.48, Tg #1 = 84.35 ± 11.65. Line 2: WT = 44.85 ± 7.294, Tg #2 = 59.51 ± 8.97. Digging: Line 1: WT = 68.41 ± 7.997, Tg #1: 59.96 ± 7.846. Line 2: WT = 16.78 ± 2.991, Tg # 2 = 14.63 ± 2.003). e, f Mice were placed in a Y-maze and allowed to freely explore the arms for 8 min. e There was no significant difference in spontaneous alternations in either line (Line 1: WT = 60.05 ± 1.889, Tg #1, 57.36 ± 2.595. Line 2: WT = 50.12 ± 3.03, Tg #2, 58.22 ± 2.919). f There was no difference in the number of arms visited (Line 1: WT = 25.57 ± 1.362, Tg #1: 23.68 ± 1.224, Line 2: WT = 23.65 ± 1.435, Tg #2: 24.67 ± 1.315. NS, not significant. *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. See Table S3 for n’s and exact statistical tests

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