Fig. 1

Creation and molecular characterization of human CYFIP1-overexpressing mice. a CYFIP1-overexpressing mice were created via pronuclear injection of a BAC containing the coding sequence for the human CYFIP1 gene. Two progeny with the highest cDNA copy number were selected to breed with wild-type C57BL6/N mice in order to establish transgenic lines 1 and 2. b hCYFIP1 copy number was confirmed using a Taqman copy number qPCR assay (Line 1 = 5.4 ± 0.34, Line 2 = 6.0 ± 0.32). c CYFIP1 mRNA expression was assessed in the cortex and hippocampus of p60 transgenic mice using qPCR. There was a significant increase in CYFIP1 mRNA expression in transgenic mice compared to their wild-type littermates (Line 1 cortex: WT = 1.03 ± 0.07, Tg #1 = 4.27 ± 0.45. Line 2 cortex: WT = 1.05 ± 0.11, Tg #2 = 3.37 ± 0.42. Line 1 hippocampus: WT = 1.00 ± 0.03, Tg #1 = 3.17 ± 0.24. Line 2 hippocampus: WT = 1.00 ± 0.03, Tg #2 = 2.10 ± 0.30). d CYFIP1 protein expression varied by region and line in p60 mice as shown by western blot analysis. The representative blot shows cortical CYFIP1 protein expression from four Line 1 WT and 4 Tg #1 animal. Line 1 had a significant increase in CYFIP1 protein expression in the cortex, but there was no detectable overexpression in the hippocampus. Line 2 showed significant CYFIP1 overexpression in the cortex but not in the hippocampus (Line 1 cortex: WT = 1.00 ± 0.80, Tg #1 = 1.94 ± 0.15. Line 2 cortex: WT = 1.05 ± 0.06, Tg #2 = 0.97 ± 0.08. Line 1 hippocampus: WT = 1 ± 0.07, Tg #1 = 1.07 ± 0.08. Line 2 hippocampus: WT = 1.00 ± 0.08, Tg #2 = 1.50 ± 0.10). P values were calculated using Student’s t test. NS, not significant. **p < 0.01. **** p < 0.0001. See Table S1 for n’s