Fig. 5

Reduction of inhibitory interneurons and morphological alteration of the mitral cell layer in Tbr1^+/− olfactory bulbs. Double immunostaining of TBR2 and a VGLUT1, b VGLUT2, c Neurofilament-light chain (NF-LC), d Calretinin, e Parvalbumin, and f Calbindin was performed to compare olfactory bulbs of Tbr1^+/− and WT mice. g Quantification of Calretinin⁺ interneurons at the glomerular layer (GL), external plexiform layer (EPL), mitral cell layer (MCL), and granular cell layer (GCL). h Quantification of parvalbumin⁺ interneurons at the external plexiform layer (EPL). i Quantification of calbindin⁺ interneurons at the glomerular layer (GL). Data represent mean plus SEM and the results of individual mice are shown. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars: 50 μm