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Table 3 Experimental conditions used to perform immunofluorescence

From: Neuroglia in the autistic brain: evidence from a preclinical model

Primary antibody Brand/cat # Dilution Secondary antibody Brand/cat #
Rabbit α-GFAP Abcam 1:200 FITC conjugated goat anti-rabbit IgG (H + L) 1:200, 5% BSA in PBS/0.25% triton X-100 Jackson ImmunoResearch
ab7260 5% BSA in PBS/0.25% triton X-100 111-095-003
Rabbit α-Olig2 Santa Cruz 1:250 FITC conjugated goat anti-rabbit IgG (H + L) 1:200, 0.5% BSA in PBS/0.25% triton X-100 Jackson ImmunoResearch
sc-48817 0.5% BSA in PBS/0.25% triton X-100 111-095-003
Rabbit α-Iba1 Wako 1:1000 FITC conjugated goat anti-rabbit IgG (H + L) 1:200, 0.5% BSA in PBS/0.25% triton X-100 Jackson ImmunoResearch
019-19741 0.5% BSA in PBS/0.25% triton X-100 111-095-003
  1. GFAP glial fibrillary acidic protein; MAP2 microtubule associated protein; BSA bovine serum albumin; FITC fluorescein isothiocyanate; PBS phosphate buffered saline