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Table 2 Experimental conditions used to perform western blot experiments

From: Neuroglia in the autistic brain: evidence from a preclinical model

Primary antibody Brand/cat # Dilution Secondary antibody Brand/cat #
Rabbit α-S100B Genetex 1:1000 HRP conjugated goat anti-rabbit IgG 1:10000 Jackson ImmunoResearch
GTX129573 5% BSA in TBS-T 0.1% 5% BSA in TBS-T 0.1% 111-035-045
Rabbit α-GFAP Abcam 1:25000 HRP conjugated goat anti-rabbit IgG 1:10000 Jackson ImmunoResearch
ab7260 5% milk in TBS-T 0.1% 5% milk in TBS-T 0.1% 111-035-045
Rabbit α-Olig2 Santa Cruz 1:500 HRP conjugated goat anti-rabbit IgG 1: 10000 Jackson ImmunoResearch
sc-48817 5% milk in TBS-T 0.1% 5% milk in TBS-T 0.1% 111-035-045
Rabbit α-CD11b Bioss 1:1000 HRP conjugated goat anti-rabbit IgG 1:10000 Jackson ImmunoResearch
bs-1014R 5% BSA in TBS-T 0.1% 5% BSA in TBS-T 0.1% 111-035-045
Rabbit α-Iba1 Abcam 1:1000 HRP conjugated goat anti-rabbit IgG 1:10000 Jackson ImmunoResearch
ab178846 5% milk in TBS-T 0.1% 5% milk in TBS-T 0.1% 111-035-045
Rabbit α-β-actin Santa Cruz 1:1000 HRP conjugated goat anti-rabbit IgG 1:20000 Jackson ImmunoResearch
sc-1616R 5% milk in TBS-T 0.1% 5% milk in TBS-T 0.1% 111-035-045
  1. GFAP glial fibrillary acidic protein; CD11b cluster of differentiation 11b; MAP2 microtubule associated protein; BSA bovine serum albumin; TBS-T tris buffered saline tween 20; HRP horseradish peroxidase