Table 1 Primer sequences and general conditions used to perform real-time qPCR
From: Neuroglia in the autistic brain: evidence from a preclinical model
GENE | Primer (5′ → 3′) | Annealing (°C) | Efficiency (%) | R 2 | |
|---|---|---|---|---|---|
S100B | Forward | TCAGGGAGAGAGGGTGACAA | 60 | 94.6 | .998 |
Reverse | ACACTCCCCATCCCCATCTT | ||||
GFAP | Forward | CGGCTCTGAGAGAGATTCGC | 60 | 105.0 | .989 |
Reverse | GCAAACTTGGACCGATACCA | ||||
Olig2 | Forward | CCCGATGATCTTTTTCTGCC | 60 | 98.8 | .990 |
Reverse | GCTTCTTATCTTTCTTGGTG | ||||
CD11b | Forward | N/A (Cod. qRnoCID0002800, Bio-Rad) | 60 | 94.0 | .990 |
Reverse | |||||
Iba1 | Forward | GTCCTTGAAGCGAATGCTGG | 60 | 95.6 | .994 |
Reverse | CATTCTCAAGATGGCAGATC | ||||
HPRT | Forward | TCCCAGCGTCGTGATTAGTGA | 60 | 98.3 | .992 |
Reverse | CCTTCATGACATCTCGAGCAAG | ||||
TBP | Forward | TGGGATTGTACCACAGCTCCA | 60 | 99.7 | .995 |
Reverse | CTCATGATGACTGCAGCAAACC | ||||