Fig. 7
From: Neuroglia in the autistic brain: evidence from a preclinical model
Effect of the prenatal VPA exposure on oligodendrocytes in adulthood. Evaluation of the transcription factor Olig2 in the PfC, Cb, and HPC of healthy- (open bars, Veh) and autistic-like (black bars, VPA) adult rats (PND 90). Representation of the relative concentration of Olig2 (a) in VPA animals compared to control (Veh), normalized to both TBP and HPRT (ΔΔCq; N = 3, in triplicate). Representative western blots for Olig2 protein and densitometric analyses normalized to β-actin used as loading control (b). Results are expressed as percentage of control (Veh) (N = 3, in triplicate). Representative fluorescence micrographs of Olig2 (green) staining in the PfC, GL and ML of Cb, and stratum radiatum of CA1, CA2, CA3, and the hilus of the DG of the HPC. Nuclei were stained with Hoechst (blue) (c). The images have been analyzed by counting the number of Olig2-positive cells in 2.4 × 105 μm3(scale bar 50 μm; N = 3, 4 times) (d). All data are presented as means ± SEM. Statistical analysis was performed by t test (*p < 0.05; **p < 0.01; ***p < 0.001 vs Veh group)