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Fig. 6 | Molecular Autism

Fig. 6

From: Autism-associated CHD8 deficiency impairs axon development and migration of cortical neurons

Fig. 6

CHD8 is required for dendrite growth in vivo. a Representative images of coronal slices of P7 mouse brains that were transfected with GFP together with indicated constructs by in utero electroporation at E14.5. Transfected cells were visualized by staining coronal slices with GFP antibody. In each image pair, the right image is from the boxed region of the left image. Scale bar, 100 μm. b Neurolucida tracings of representative layer II/III pyramidal neurons, from experiment shown in a. Scale bar, 50 μm. ce Quantitative analysis of dendrite length and dendrite complexity measured from Neurolucida tracings. Mean dendritic length: shRNA-scr, 109.2 ± 7.5 μm; shRNA#1, 85.9 ± 5.59 μm, *P = 0.015, shRNA#1 vs shRNA-scr. Total dendritic length: shRNA-scr, 541.6 ± 47.52 μm; shRNA#1, 415.233.89 μm, *P = 0.0332, shRNA#1 vs shRNA-scr. Greater than 70 neurons were examined in each group from at least three independent experiments. Results are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; one-way ANOVA post Dunnett’s test

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