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Fig. 3 | Molecular Autism

Fig. 3

From: Autism-associated CHD8 deficiency impairs axon development and migration of cortical neurons

Fig. 3

Cell type-specific expression pattern of CHD8 in mouse brains. a Endogenous mouse CHD8 is selectively enriched in the nucleus of cultured DIV3 cortical neurons. Cells were immunolabeled with CHD8 (green), the somatodendritic marker MAP2 (red), and with counterstaining of nuclear marker Hoechst (blue). Scale bar, 2 μm. b Double-immunofluorescent staining shows that CHD8 is highly expressed in NeuN-positive neurons. Scale bar, 5 μm. The open arrows indicate both CHD8 and NeuN-positive neurons, while the filled arrows indicate neither CHD8 nor NeuN-positive non-neuronal cells. c Double-immunofluorescent staining shows that CHD8 is weakly expressed in GFAP-positive astrocytes. The open arrows indicate GFAP-positive astrocytes with weak CHD8 expression. Scale bar, 25 μm. d Representative western blot shows that CHD8 is predominately expressed in neurons but also in cultured glia cells

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