A behavioral test battery for mouse models of Angelman syndrome: a powerful tool for testing drugs and novel Ube3a mutants

Table 2 Achieved power for each behavioral test of the behavioral test battery

	Wild type (mean ± SD)	Ube3a (mean ± SD)	Test	Achieved effect size	Sample size per group (1-β)= 0.95	Sample size per group (1-β) = 0.90	Sample size per group (1-β) = 0.80
Rotarod Time on machine (s)	149 ± 55	95 ± 40	ANOVA	0.56	14	11	9
Open field Distance moved (m)	40 ± 13	26 ± 13	T test	1.17	21	17	13
Marble burying (# marbles buried)	11 ± 4	4 ± 4	T test	2.26	7	6	5
Nest building (% used nesting material)	68 ± 23	28 ± 19	T test	1.95	8	7	6
Forced swim test (% floating time)	37 ± 25	73 ± 18	T test	1.73	10	9	7
Susceptibility to audiogenic seizure (% of animals)	7	98	T test	4.55	3	3	3

Data provided is based on the experiments using Ube3a^tm1Alb mice in F1 hybrid 129S2-C57BL/6J background. The table provides the obtained effect size, number of mice needed per genotype for each behavioral test (with power equal to 0.95, 0.90, 0.80), and statistical test used. For rotarod calculations, we used the average performance over the 5 days, while for the nest building we used the data of the last test day

ISSN: 2040-2392