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Fig. 1 | Molecular Autism

Fig. 1

From: Characterization and structure-activity relationships of indenoisoquinoline-derived topoisomerase I inhibitors in unsilencing the dormant Ube3a gene associated with Angelman syndrome

Fig. 1

Like the camptothecin-derived topotecan, indenoisoquinoline derivatives can unsilence paternal Ube3a-YFP in vitro. a Immunofluorescence images of nuclei (Hoeschst stain) and paternal UBE3A-YFP in drug-treated cultured mouse cortical neurons and chemical structures of the compounds. Paternal Ube3a-YFP was unsilenced by the indicated drugs [topotecan (0.3 μM), indotecan (0.3 μM), indimitecan (0.3 μM), DB-IV-58 (0.3 μM), or DB-V-37 (0.3 μM)] but not by DMSO vehicle control (scale bar = 100 μm). DB-IV-58 and DB-V-37 are structural analogues of indotecan and indimitecan. b Quantitative analysis of neurons expressing unsilenced paternal UBE3A-YFP (n = 4 wells in 384-well plate/group, *p < 0.05)

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