Integrated genome-wide Alu methylation and transcriptome profiling analyses reveal novel epigenetic regulatory networks associated with autism spectrum disorder

Table 7 Quantitative RT-PCR analyses of AluS expression levels in the LCLs of ASD and control groups

Group	Fold change (FC)	Log₂ (FC)	P value
ASD vs. control	1.75	0.81	0.316
Subgroup M vs. control	1.05	0.06	0.953
Subgroup L vs. control	0.29	− 1.77	0.032
Subgroup S vs. control	3.68	1.88	0.038

The levels of Alu transcripts were normalized to the housekeeping gene GAPDH. The AluS expression levels were calculated using the 2^−ΔΔCt method, and differences with a P value < 0.05, as determined by two-tailed t tests with Benjamini-Hochberg correction, were considered significant

ISSN: 2040-2392