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Table 6 COBRA-derived percentages of AluS methylation and patterns in LCLs from ASD individuals and sex- and age-matched controls

From: Integrated genome-wide Alu methylation and transcriptome profiling analyses reveal novel epigenetic regulatory networks associated with autism spectrum disorder

Comparison

Sample groups

Age (mean ± SD)

Percentages of AluS methylation patterns

%mC

%mCmC

%uCmC

%mCuC

%uCuC

ASD vs. control (sex- and age-matched)

Control (n = 20)

15 ± 6.97

37.98 ± 1.36

25.70 ± 2.36

18.71 ± 1.15

21.76 ± 1.03

33.83 ± 1.69

ASD (n = 36)

13.4 ± 4.55

37.86 ± 2.07

25.92 ± 2.69

18.72 ± 1.66

21.22 ± 1.76

34.13 ± 3.00

P value

0.866

0.866

0.974

0.315

0.845

Subgroup M vs. control (sex- and age-matched)

Control (n = 10)

12.1 ± 3.81

38.07 ± 1.52

25.57 ± 2.31

18.76 ± 0.87

22.06 ± 0.69

33.62 ± 1.85

ASD subgroup M (n = 10)

12.1 ± 3.73

39.03 ± 1.16

25.17 ± 1.15

20.06 ± 0.92

22.99 ± 0.82

31.77 ± 1.76

P value

0.293

0.845

0.043

0.089

0.168

Subgroup L vs. control (sex- and age-matched)

Control (n = 6)

13.7 ± 1.64

37.84 ± 1.51

24.88 ± 2.94

19.10 ± 1.60

22.25 ± 1.03

33.77 ± 1.84

ASD subgroup L (n = 6)

13.5 ± 1.97

36.024 ± 1.43

23.37 ± 2.63

19.32 ± 1.69

20.93 ± 2.52

36.38 ± 2.29

P value

0.168

0.569

0.866

0.502

0.168

Subgroup S vs. control (sex- and age-matched)

Control (n = 20)

15 ± 6.97

37.98 ± 1.36

25.70 ± 2.36

18.71 ± 1.15

21.76 ± 1.03

33.83 ± 1.69

ASD subgroup S (n = 20)

15 ± 5.41

37.83 ± 2.23

27.06 ± 2.67

17.88 ± 1.44

20.43 ± 1.17

34.64 ± 3.00

P value

0.866

0.242

0.168

0.010

0.502

  1. The percentages of AluS methylation patterns were determined based on four patterns: the hypermethylated pattern (mCmC), two partially methylated patterns (mCuC, uCmC), and the hypomethylated pattern (uCuC). Comparisons of the methylation status between ASD and sex- and age-matched unaffected control groups and between ASD phenotypic subgroups and the matched unaffected controls were also performed. Statistically significant P values < 0.05 with Benjamini-Hochberg correction (FDR = 0.05) are shown in italics