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Fig. 3 | Molecular Autism

Fig. 3

From: CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors

Fig. 3

shank3b−/− larvae displayed impaired locomotion activity. a–c Spontaneous activity of shank3b+/+, shank3b+/−, and shank3b−/− larvae was significantly reduced at 5 and 7 dpf, but not at 2 dpf. The X axis shows the intensity scale of the activity and Y axis shows the normalized activity frequency traveled by larvae in 1-min bin on each intensity scale (N = 24 for each genotype). df’ Light/dark test of shank3b+/+, shank3b+/−, and shank3b−/− larvae at 5 and 7 dpf. The activity was recorded during 30 min of light (L0) and three 5-min light/dark intervals (D1/L1, D2/L2, and D3/L3) (d). The average distance moved within each 1-min bin under either light or dark conditions is plotted. Experiments were performed at 5 dpf (e and e’) and 7 dpf (f and f’). The vertical axis shows the normalized distance (millimeters) traveled by larvae in each 1-min bin. Data are shown as mean ± SEM (N = 24 for each genotype); *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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