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Fig. 1 | Molecular Autism

Fig. 1

From: CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors

Fig. 1

Generation of shank3b mutation in zebrafish by CRISPR-Cas9 gene editing. a Structure of zebrafish shank3b gene and protein. The protein domains (ANK, ankyrin repeat domain; SH3, Src homology 3 domain; PDZ, PSD-95/Discs large/ZO-1 domain; SAM, sterile alpha motif domain) are aligned to the corresponding exons. Exon 2 is the target for CRISPR/Cas9 gene editing in zebrafish shank3b. The CRISPR/Cas9-induced mutation (5-base deletion and 13-base insertion) in shank3b is shown in annotated shank3b mutant sequences. The nucleotides in red are inserted sequences and the green highlighted “-” are deleted nucleotides. b Reduced expression of shank3b mRNA in the brain of shank3b+/+ and shank3b−/− adult (6 mpf) male zebrafish analyzed by RT-qPCR. c The expression of shank3a mRNA in the brain of shank3b+/+ and shank3b−/− adult (6 mpf) male zebrafish was not affected. Data are shown as mean ± SEM; ***p < 0.001

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