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Fig. 1 | Molecular Autism

Fig. 1

From: 17-β estradiol increases parvalbumin levels in Pvalb heterozygous mice and attenuates behavioral phenotypes with relevance to autism core symptoms

Fig. 1

Western blot analysis and RT-qPCR of forebrain samples from PV+/+ and PV+/− mice either vehicle-treated (−) or E2-treated (+) from PND5-15. a Left: representative Western blots of PV (Mr: 12 kDa) and GAPDH (Mr: 35 kDa; normalization signal) are shown. Middle: quantitative analysis of PV signals in mice treated with 10 μg E2/day/pup. Data are obtained from three independent experiments and are shown as mean ± SEM. Results are expressed as a percentage of normalized PV levels as measured in vehicle-treated PV+/+ samples (set as 100%). GAPDH was used for the normalization of the PV signals. Right: RT-qPCR analysis of forebrain samples of PV+/+ and PV+/− mice. Pvalb mRNA levels were normalized to 18S mRNA levels and expressed as fold change. Data from three independent experiments were pooled and are shown as mean ± SEM. b Representative Western blots (left) and quantitative analysis of PV signals from samples derived from mice treated with 50 μg E2/day/pup (right). PV signal quantification was done as in a. Asterisks represent *p < 0.05, **p < 0.01, respectively

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