Fig. 2

Prenatal levonorgestrel exposure induces ERβ suppression in amygdala neurons through increased methylation on the ERβ promoter, while EE has no effect. a The amygdala neurons were isolated from 10-week-old male/female offspring for in vitro cell culture analysis. The representative bands for ERβ methylation in amygdala neurons from both male (upper panel) and female (lower panel) offspring. b DNA methylation on ERβ by real-time PCR-based methylation-specific PCR (MSP) analysis in amygdala neurons, n = 5. c ChIP analysis on the ERβ promoter in male amygdala neurons, n = 5. d ChIP analysis on ERβ promoter in female amygdala neurons, n = 5. *P < 0.05 vs. VEH group; P < 0.05 vs. LNG group. Results are expressed as mean ± SEM