Fig. 3

Results of Western blot and immunofluorescence from MeCP2 WT and mutant expression in vitro. a The example picture of mouse primary cortical neurons transfected (0 DIV) with GFP alone and GFP with constructs MECP2 mutant or wild type at 3 DIV. “Total length of neurons,” which means the length of all dendritic branches (including secondary branch) add the axon (include sub-axon). b Identification of MeCP2 expression by Western blots in each condition: GFP (null), MeCP2-WT (70~100 KD), MeCP2-P152L (70~100 KD), MeCP2-R294X (40~55 KD), MeCP2-P376S (70~100 KD), β-actin (≈40 KD). c–e The length of total neurites, the longest axon, and total dendrite of each condition. *p < 0.05, **p < 0.01, ***p < 0.001 (t test). All neurons were fixed at 3 DIV and immunostained with GFP antibody for measurement. A total 40–50 neurons from each condition were randomly selected and measured via blind method