Fig. 4
From: Prenatal exposure to valproic acid increases miR-132 levels in the mouse embryonic brain
Effects of prenatal VPA exposure at E14.5 on miR-132 levels in the male and female mouse embryonic whole brain. Embryos were obtained from mothers treated with VPA (500 mg/kg, i.p.) or saline at E12.5, 6 h after the drug exposure, and whole brains were immediately removed from the embryos. Total RNA was extracted from the brains and subjected to real-time PCR analysis. The results, which are normalized to GAPDH mRNA levels, are expressed as % of saline-treated control, and are presented as means ± SEM (n = 4 from two VPA and one saline-treated dams)