Fig. 6

Social interactions. Shank3B male null mutant mice (KO) interacting with an estrus WT female displayed deficits on some parameters of reciprocal social interactions, as compared to wildtype littermate males (WT), replicated in two independent cohorts. Time spent in nose-to-anogenital sniffing over the 5-min test session was less in Shank3B KO than WT (a cohort 1, *p < .05; e cohort 2, *p < .05). Time spent in nose-to-nose sniffing was not significant in cohort 1 (b), but reached significance in cohort 2 (*p < .01). Ultrasonic vocalizations emitted during the 5-min male-female interaction sessions were lower in KO than WT in both cohorts on total number of calls (c cohort 1, *p < .02; g cohort 2, *p < .02) and on calls per minute (d cohort 1, *p < .01; h cohort 2, *p < .02)