Fig. 2

Analysis of PTZ-induced seizures in cohort 1 (a-c) and in cohort 2 (d-f). a and d Incidence and latency to first myoclonus after PTZ injection. Kaplan–Meier survival curve is used to display percentage incidence of first myoclonus (y-axis) and its latency (x-axis), after PTZ injection (40 mg/kg). Curve comparison in first cohort shows that the incidence of myoclonus rate of (100%) in WT group was significantly higher than in the Shank3B KO group (67%) in a, which was replicable in the second cohort with only 50% knockouts experiencing a myoclonus in comparison to 100% of WT mice in d. Moreover, the Shank3B KO mice had a significantly longer latency to the first myoclonic seizure relative to WT controls. b and e Myoclonic seizure count. The frequency of PTZ-induced myoclonic seizures per 20 min of recording was significantly reduced in Shank3B KO mice relative to WT as replicated in two separate cohorts (b for cohort 1 and e for cohort 2) . c and f Epileptic spike count. The number of epileptic spikes on the EEG was also significantly lower in Shank3B KO mice relative to WT mice in both cohorts (c for cohort 1 and f for cohort 2). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001