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Fig. 4 | Molecular Autism

Fig. 4

From: Increased expression of the PI3K catalytic subunit p110δ underlies elevated S6 phosphorylation and protein synthesis in an individual with autism from a multiplex family

Fig. 4

Western blot analyses confirm increased S6 phosphorylation in select SSC cell lines compared to their unaffected siblings. S6 phosphorylation was significantly increased in cell line S1 (a, n = 3, paired t test, t(2) = 10.7, p = 0.009) and S11 (b, n = 4, paired t test, t(3) = 10.39, p = 0.002) but not in S3 (c, n = 4, paired t test, t(3) = 0.72, p = 0.525), confirming ELISA results shown in Fig. 2. Example Western blots are shown on the top and densitometric quantification of three to four independent experiments below. Phosphorylated S6 was normalized to tubulin. Bar diagrams are means + SEM. None of the class IA PI3K catalytic subunits p110α, p110β, or p110δ was significantly different in S1 or S11 compared to their unaffected siblings (see Additional file 3: Figure S2)

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Molecular Autism

ISSN: 2040-2392