Fig. 1

Phospho-S6- and S6-specific ELISAs on lymphoblastoid cell lines from patients with idiopathic autism from AGRE. a Phosphorylation of S6 is significantly increased in three cell lines (A1, A4, and A5) (one-way ANOVA F(21,85) = 3.5; p < 0.0001, Dunnett’s post hoc comparisons of autism cell lines to the unaffected control (CTR) *p(A1) = 0.041, *p(A4) = 0.011, *p(A5) = 0.015). b Expression of total S6 (phosphorylated and unphosphorylated) shows much less variability than phospho-S6. Two of the three cell lines with increased S6 phosphorylation also have increased S6 levels (A1 and A5) (one-way ANOVA F(21,85) = 5.468; p < 0.0001, Dunnett’s post hoc comparisons of autism cell lines to the unaffected control *p(A1) = 0.013, *p(A5) = 0.0003). c Phospho-S6/S6 ratios were significantly increased in cell line A4, suggesting an upstream signal transduction defect (one-way ANOVA F(21,85) = 2.34; p = 0.003, Dunnett’s post hoc comparisons of autism cell lines to the unaffected control *p(A4) = 0.014). N = 4, shown are means + SEM. List of p values for all pairwise comparisons in Additional file 2: Table S1a. Further control experiments are shown in Additional file 1: Figure S1a–c