Fig. 4

Effects of mRbfox1-iso2-silencing on the cell cycle of VZ/SVZ progenitor and stem cells. a Effects of mRbfox1-iso2-silencing on EdU incorporation were examined. Cortices were electroporated in utero with pCAG-H2B-EGFP together with pSuper vector (control) or pSuper-mRbfox1-iso2 (iso2). Coronal sections were visualized for GFP (green) and EdU (red). Arrowheads indicate EdU/GFP double positive cells. Dotted lines represent ventricular surface. Bar, 5 μm. b Quantification of EdU/GFP double positive cells among GFP-positive ones (n = 100) in a. Two sections were used per brain (control, n = 5; iso2, n = 4). Error bars indicate SD. c Effects of mRbfox1-iso2-silencing on cell cycle exit. Differentiated neurons are EdU/GFP double positive while EdU/Ki67/GFP-triple-positive cells maintain progenitor potency. Arrowheads indicate triple-positive cells. d Quantification of EdU/Ki67/GFP-triple-positive cells among GFP/EdU double positive ones (n = 100) in c. The ratio of the triple-positive cells over the total double positive ones in mRbfox1-iso2-silencing experiments was similar to that in the control ones. Two sections were used per brain. Error bars indicate SD (control, n = 3; iso2, n = 3)