CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment

Table 2 Selected differentially expressed genes associated with ASD risk

Gene	Coordinate	ASD score			CHD8-binding in NPCs	CHD8 ^+/− NPC		CHD8 ^+/− neuron
Gene	Coordinate	SFARI	AutismKB	Willsey	CHD8-binding in NPCs	log2(FC)	q value	log2(FC)	q value
ANK2	chr4:113739265-114304896	High confidence	9	hcASD	N	−1.87	1.36E-45	−1.96	6.32E-08
SETD5	chr3:9439299-9520924	High confidence	10		Y	0.25	0.67	0.87	0.00053
SUV420H1	chr11:67922330-67981295	High confidence	16	hcASD	Y	−0.084	0.89	−0.86	0.0020
SCN2A	chr2:166095912-166248818	High confidence	20	hcASD	N	−0.64	0.86	−3.31	5.67E-05
DEAF1	chr11:644233-706715	Strong candidate	2		N	−0.16	0.79	−0.68	0.0010
MYT1L	chr2:1792885-2335032	Strong candidate	2		N	0.67	0.72	−1.76	0.0077
BCL11A	chr2:60678302-60780702	Strong candidate	9		N	1.91	0.029	0.25	0.61
CNTN4	chr3:2140497-3099645	Strong candidate	9		N	6.02	0.53	5.15	1.60E-14
CACNA2D3	chr3:54156574-55108584	Strong candidate	10	pASD	Y	2.64	0.0036	−2.95	0.00076
CACNA1H	chr16:1203241-1271771	Strong candidate	10		Y	0.75	0.045	−0.19	0.58
NRXN1	chr2:50145643-51259674	Strong candidate	28	pASD	Y	−0.81	0.77	−1.71	2.13E-05
MET	chr7:116312444-116438440	Strong candidate	33		N	0.69	0.82	−3.56	3.45E-05
GABRB3	chr15:26788693-27184686	Strong candidate	34		N	−0.43	0.66	−1.70	0.00030
RELN	chr7:103112231-103629963	Strong candidate	43	pASD	N	1.82	0.16	−2.92	1.46E-06

ISSN: 2040-2392