NTRK2 expression levels are reduced in laser captured pyramidal neurons from the anterior cingulate cortex in males with autism spectrum disorder

Table 1 Subject demographic information

Pair	ID	Age	Gender	RIN ^a	PMI (hours) ^b	Toxicology	Assays	Tissue
Control donors
1	AN14757	24	M	7.8	21.33	No drugs reported	LCM^c, qPCR^d	BA24, BA10
2	AN07176	21	M	8	29.91	No drugs reported	LCM, qPCR	BA24, BA10
3	AN07444	17	M	7.5	30.75	Sertraline	LCM, qPCR	BA24
4	5408	6	M	7	16	No drugs reported	LCM, qPCR	BA24, BA10
5	4848	16	M	7.6	15	No drugs reported	LCM, qPCR	BA24, BA10
6	5342	22	M	8.1	14	No drugs reported	LCM, qPCR	BA24, BA10
7	5079	33	M	7.3	16	Ethanol	LCM, qPCR	BA24, BA10
8	M3231M	37	M	7.4	24	No drugs reported	LCM, qPCR	BA24, BA10
9	4590	20	M	7.6	19	No drugs reported	LCM, qPCR	BA10
Mean ± SEM (BA24 donors)		22.0±3.4		7.59±0.13	20.9±2.4
Mean ± SEM (BA10 donors)		22.4±3.4		7.60±0.13	19.4±1.9
ASD donors
1	AN04166	24	M	8.1	18.51	No drugs reported	LCM, qPCR	BA24, BA10
2	AN03935	20	M	8.6	28	No drugs reported	LCM, qPCR	BA24, BA10
3	AN02987	15	M	6.5	30.83	No drugs reported	LCM, qPCR	BA24
4	5144	7	M	8.0	3	No drugs reported	LCM, qPCR	BA24, BA10
5	5302	16	M	6.6	20	Risperidone, fluvoxamine, clonidine, insulin	LCM, qPCR	BA24, BA10
6	5176	22	M	7.1	18	Risperidone	LCM, qPCR	BA24, BA10
7	5297	33	M	7.1	50	Quetiapine, fluoxetine, valproate, ziprasidone	LCM, qPCR	BA24, BA10
8	5027	37	M	7.7	26	Risperidone, fluvoxamine	LCM, qPCR	BA24, BA10
9	4999	20	M	7.0	14	No drugs reported	LCM, qPCR	BA10
Mean ± SEM (BA24 donors)		21.6±3.4		7.46±0.27	24.3±4.8
Mean ± SEM (BA10 donors)		22.2±3.3		7.53±0.24	23.4±5.4
P value ^e (BA 24)		0.94		0.68	0.53
P value ^e (BA10)		0.98		0.79	0.60

^aRNA integrity number (index of RNA quality); ^bpostmortem interval; ^cendpoint PCR analysis of reversed transcribed RNA isolated from cells collected by laser capture microdissection; ^dquantitative polymerase chain reaction of reverse transcribed RNA isolated from punch-dissected tissues; ^eresults of a two-tailed independent t-test comparing control and ASD groups. BA24, Brodmann area 24; BA10, Brodmann area 10; LCM, laser capture microdissection.

ISSN: 2040-2392