Laser capture microdissection of pyramidal neurons and astrocytes. Shown in (A) is the absence of laser captured nissl-stained pyramidal neurons from cortical layer 3 in BA24 gray matter tissue following capture (20× magnification). (B) shows those cells adhered to the polymer cap (20× magnification). (C) and (D) illustrate the before (C) and after (D) capture images for nissl-stained BA24 pyramidal neurons (40× magnification). (E) and (F) show the same laser capture process for GFAP immunostained astrocytes in BA24 gray matter (60× magnification).