Co-localization of wild-type GPR85 or GPR85 mutants and PSD-95 in neurons. Co-localization of wild-type GPR85, GPR85(M152T) or GPR85(V221L), and PSD-95 was examined in hippocampal neurons (DIV 9) at 2 days after transfection. Red, wild-type GPR85 and GPR85 mutants (M152T and V221L)-Myc-His; green, PSD-95-GFP. Scale bars, 20 μm. Closed arrowheads, punctum of GPR85 co-localized with PSD-95 at synapse; open arrowheads, punctum of GPR85 not co-localized with PSD-95 at synapse. PSD, postsynaptic density protein.