mutations detected and family trees of the patients with each base substitution. (a) M152T in GPR85 of patient MR38 and his family member. (b) V221L in GPR85 of patient Aut59 and his family member. NA, not available. (c) The position of two mutations, M152T and V221L, in the human GPR85 molecule. Arrows indicate the position of M152T (red) and V221L (yellow).