Figure 1

Zn ²⁺ partially reverses the hDAT T356M deficits in [ ³ H]DA uptake and amphetamine (AMPH)-mediated efflux. Methods were as previously described in Hamilton et al. [5]. (A) [³H]DA uptake counts (cpm) are plotted for hDAT and hDAT T356M over a range of Zn²⁺ concentrations. While Zn²⁺ inhibits hDAT [³H]DA uptake, Zn²⁺ instead increases hDAT T356M [³H]DA uptake (*p < 0.05 by one-way ANOVA followed by Dunnett’s test compared to 0 Zn²⁺ control; n = 4). (B) (Top) Representative AMPH-induced amperometric currents (reflecting DA efflux) are displayed in the presence or absence of 100 μM Zn²⁺. Arrows indicate the application of 10 μM AMPH. (Bottom) Maximal DA efflux amperometric current recorded in the presence of Zn²⁺ normalized to maximal current recorded in the presence of vehicle. (*p < 0.05 by paired Student’s t-test; n = 5). (C) (Top) Representative Zn²⁺-induced change in amperometric currents are displayed in response to 100 μM Zn²⁺ or vehicle control. Arrows indicate the application of 100 μM Zn²⁺. (Bottom) Change in amperometric current recorded in response to 100 μM Zn²⁺ or vehicle control (*p < 0.05 by paired Student’s t-test; n = 5).