Figure 1

RORA and aromatase protein in the postmortem frontal cortex (BA9) of females and males. Comparison of RORA (A) and aromatase (B) protein levels in postmortem tissues from the frontal cortex of control females (n = 8) and age-matched control males (n = 9) as well as age-matched females (n = 5) and males (n = 4) with ASD. Results were obtained by re-analysis of data from confocal immunofluorescence analyses of tissue arrays previously reported by Sarachana et al. [30]. The dark bars in the boxes represent the medians, the box limits indicate the 25th and 75th percentiles as determined by the statistical software R, and the whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. The open circle above the boxplot represents an outlier in the aromatase level for control males. The female-to-male (F/M) protein ratios (shown in C) were calculated based on the average mean fluorescence values for the respective groups, and two-tailed P values, t-values, and effect sizes are given for the comparisons between the protein levels in females and males.