Decrease in mRNA levels of ER co-activators (SRC-1, CBP, and P/CAF), but no change in ER co-repressors (SMRT and nCoR) in the middle frontal gyrus of ASD subjects. ( A–E ) Normalized mRNA expression of ER co-activators: ( A ) SRC-1, ( B ) CBP, ( C ) P/CAF, ( D ) TIF-2, and ( E ) AIB-1. ( F and G ) Normalized mRNA expression of ER co-repressors: ( F ) SMRT and ( G ) nCoR. mRNA levels were determined by qRT-PCR, and the values were normalized to the geometric mean of two control genes (GAPDH and β-actin). ***P <0.001 vs. controls.