α1 protein, but not mRNA expression in the middle frontal cortex of ASD subjects. (A) GABAAα1 protein levels were determined by western blot analysis. The upper panel shows representative autoradiogram of GABAAα1 or GAPDH, and the lower panels represent fold change in normalized GABAAα1 protein levels. Results are mean ± SEM. *P <0.05 vs. controls. (B-D) Representative autoradiogram and fold change in protein levels of (B) GABAAα2, (C) GABAAα3, and (D) GABAAγ2 in ASD and control subjects. (E) mRNA was determined by qRT-PCR, and the values were normalized to the geometric mean of two control genes (glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and ß-actin).