Cell line | % methylated at PWS-ICa |
---|
mat. Int dup(15) |
66.57 ± 0.92
|
mat. Int dup(15)-02 | 66.15 ± 2.12 |
mat. Int dup(15)-12b | 65.64 ± 3.25 |
pat. Int dup(15) |
25.31 ± 1.46
|
pat. Int dup(15)-04 | 23.53 ± 2.93 |
Idic
|
76.94 ± 5.02
|
Idic1-8 | 72.43 ± 1.00 |
IdicCB
|
77.35 ± 3.52
|
IdicCB-07 | 79.49 ± 5.03 |
IdicCB-09 | 72.29 ± 9.69 |
- aDNA methylation at the PWS-IC was assayed by methylation specific qPCR using genomic DNA from Dup15q iPSCs. The average calculated percent methylation from all clones within a genotype is presented plus or minus the standard deviation in bold text. The averages calculated for percent methylation from three replicate assays each for selected clones plus or minus the standard deviation are shown in plain text. bThe mat. int dup(15)-12 cell line was originally identified as an isogenic normal clone derived from the individual with maternal interstitial duplication. However, methylation analysis after approximately 25 subsequent passages suggests that this clone was mosaic and resolved to contain predominantly duplication containing cells. PWS-IC, Prader-Willi syndrome imprinting center; mat. Int dup(15), maternal interstitial duplication of 15q11-q13.1; pat. Int dup(15), paternal interstitial duplication of 15q11-q13.1; Idic, isodicentric chromosome 15; IdicCB, isodicentric chromosome 15 cord blood derived sample.