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Figure 7 | Molecular Autism

Figure 7

From: Gene expression analysis of human induced pluripotent stem cell-derived neurons carrying copy number variants of chromosome 15q11-q13.1

Figure 7

Rescue of normal UBE3A expression levels following treatment of isodicentric chromosome 15 (idic(15)) induced pluripotent stem cell (iPSC)-derived neurons with mithramycin. (a) qRT-PCR analysis of UBE3A mRNA expression in 10-week-old idic(15) (Idic1-8) iPSC-derived neurons following 72 hours of treatment with DMSO (vehicle) or mithramycin at concentrations ranging from 25 nM to 1 μM. Data are presented as mean expression levels relative to that of untreated normal (Nml 1-0) iPSC-derived neurons. (b) ChIP-qPCR analysis of YY-1 binding at two sites in UBE3A, one within exon 1 and one upstream of exon 1, in 10-week-old idic(15) (Idic1-8) iPSC-derived neurons following 72 hours of treatment with either DMSO or 100 nM mithramycin.

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Molecular Autism

ISSN: 2040-2392