Quantitative reverse transcription PCR (qRT-PCR) validation of mRNA-Seq data. (a) Differential gene expression of select autism candidate genes and genes implicated in seizure disorders were verified by qRT-PCR in 10-week-old AS (AS del 1-0), normal (Nml 1-0), and idic(15) (Idic1-8) iPSC-derived neurons. P values: *P ≤0.1, **P ≤0.05, ***P ≤0.01. (b) Correlation of relative expression levels for 19 genes in iPSC-derived neurons as determined by mRNA-Seq and qRT-PCR. The log2-transformed RQ values for mRNA expression relative to normal neurons are plotted on the x-axis against the log2-transformed FPKM values obtained by mRNA-Seq analysis (y-axis).