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Figure 3 | Molecular Autism

Figure 3

From: N-ethylmaleimide-sensitive factor interacts with the serotonin transporter and modulates its trafficking: implications for pathophysiology in autism

Figure 3

Knockdown efficiency of NSF siRNAs and effect on expression level of SERT in HEK293-hSERT cells. Two siRNAs targeting specific NSF sequences were transfected into HEK293-hSERT cells. (A) The expression levels of NSF and β-actin (as an internal control) were assayed by immunoblot analysis. (B) Quantitation of relative band densities for NSF was performed by scanning densitometry. Data are expressed as the means ± standard deviation, n = 3. ***P < 0.001 vs internal control (one-way ANOVA with Tukey’s post hoc test). (C) The expression levels of SERT and β-actin (as an internal control) were assayed by immunoblot analysis. (D) Quantitation of relative band densities for SERT was performed by scanning densitometry. Data are expressed as the means ± standard deviation, n = 5 or 6. NSF, N-ethylmaleimide-sensitive factor; SERT, serotonin transporter; siRNA, small interfering RNA.

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Molecular Autism

ISSN: 2040-2392