NSF co-localizes with SERT in HEK293-hSERT cells. (A,B,C) Double immunocytochemical staining for SERT (green) and NSF (red) in HEK293-hSERT cells. NSF co-localizes with SERT in the cell membrane (merged). (D,E, F) High-magnification views of the regions boxed in panels (A), (B) and (C), respectively. Arrowheads indicate double-positive intracellular particles. Scale bar: 10 μm. Results are representative of three independent experiments. NSF, N-ethylmaleimide-sensitive factor; SERT, serotonin transporter.