Analysis of MIPS validation experiment. (A) First 44 genes with prior de novo mutations were sequenced for 2,448 additional trios. These genes were cross-classified by whether or not they had a prior dnLoF mutation, and whether or not they were on the DAWN rASD list (yes: red, no: blue). For each category, the percentage of genes that had a dnLoF mutation in the new trios is depicted.(B) For a given gene, the probability of observing a dnLoF mutation in 2,500 probands varies. This probability is compared for four types of genes: a randomly chosen gene and three classifications of the genes from the MIPS experiment including: (i) all 44 genes, (ii) those 16 genes with a prior dnLoF mutation and (iii) those 10 genes on the rASD list. dnLoF, de novo loss of function; rASD, risk autism spectrum disorder.