Table 1 Proteins identified by tandem mass spectrometry following two-dimensional DIGE analysis
From: Protein signatures of oxidative stress response in a patient specific cell line model for autism
| Â | RPL10 vs Ctrl | ASD vs CTRL | MS/MS results | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Name a | Full name | Spot No. | FC | T-test sig. | FC | T-test sig. | F-test sig. | Cov. | Prot. Score | Comment | Network |
ACTB | Actin, beta | 65 | 1.032 | >0.1 | −1.184 | 0.0127 | 0.0949 | 55% | 216 | Confirmed twice | NA |
ALDOC | Aldolase C, fructose-bisphosphate | 57 | −1.375 | 0.0932 | −1.277 | 0.0018 | >0.1 | 44% | 125 | Mixture with PCBP1 | Energy metabolism |
ATP5A1 | ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit 1 | 70 | 1.165 | >0.1 | −1.295 | 0.0289 | >0.1 | 32% | 153 | Confirmed twice; mixture with GLUD1 | Energy and redox metabolism |
ATP5B | ATP synthase, H + transporting, mitochondrial F1 complex, beta polypeptide | 61 | 1.087 | >0.1 | 1.151 | 0.0073 | >0.1 | 16% | 48 | - | Energy and redox metabolism |
ATP5H | ATP synthase, H + transporting, mitochondrial F0 complex, subunit d | 77 | 1.036 | >0.1 | −1.013 | >0.1 | 0.0896 | 67% | 134 | Confirmed twice | Energy and redox metabolism |
ECH1 | Enoyl Coenzyme A hydratase 1, peroxisomal | 9 | 1.762 | 0.0003 | −1.690 | >0.1 | >0.1 | 27% | 46 | - | Energy metabolism |
ECHS1 | Enoyl Coenzyme A hydratase, short chain, 1, mitochondrial | 59 | −1.016 | >0.1 | −1.405 | 0.004 | >0.1 | 12% | 43 | Confirmed twice | NA |
EEF1D | Eukaryotic translation elongation factor 1 delta | 75 | 1.285 | 0.0085 | −1.078 | >0.1 | 0.0429 | 27% | 71 | - | Protein and mRNA metabolism |
ENOA | Alpha-enolase | 20 | −1.058 | >0.1 | −1.068 | >0.1 | >0.1 | 33% | 81 | Picked as potential housekeeper | NA |
ERP29 | Endoplasmic reticulum protein 29 | 58 | 1.258 | >0.1 | 2.287 | 0.0024 | >0.1 | Â | 1 Protb | - | Protein and mRNA metabolism |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 18 | −1.453 | 0.0528 | −1.005 | >0.1 | >0.1 |  | 1 Protb | - | Energy and redox metabolism |
18c | −1.061 | >0.1 | 1.078 | 0.0294 | >0.1 | 59% | 191 | - |  | ||
GLUD1 | Glutamate dehydrogenase 1 | 70 | 1.165 | >0.1 | −1.295 | 0.0289 | >0.1 | 20% | 78 | Confirmed twice; mixture with ATP5A1 | Redox metabolism |
HNRNP A2B1 | Heterogeneous nuclear ribonucleoprotein A2/B1 | 71 | −1.242 | 0.0796 | 1.173 | 0.0298 | 0.0391 | 58% | 196 | Confirmed twice | Protein and mRNA metabolism |
18b | 1.008 | >0.1 | 1.100 | 0.0286 | >0.1 | 45% | 104 | Confirmed twice | |||
HNRNPK | Heterogeneous nuclear ribonucleoprotein K | 14 | −2.792 | 0.0017 | −1.209 | 0.0955 | 0.0003 | 13% | 36 | - | Protein and mRNA metabolism |
HNRPDL | Heterogeneous nuclear ribonucleoprotein D-like | 80 | −1.037 | >0.1 | 1.073 | >0.1 | 0.0323 | 19% | 78 | Confirmed twice | Protein and mRNA metabolism |
HSPA5 | Heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa) | 21 | −1.130 | >0.1 | 1.004 | >0.1 | 0.0589 | 21% | 94 | - | Protein and mRNA metabolism |
HSPD1 | Heat shock 60Â kDa protein 1 | 74 | 1.357 | >0.1 | 1.130 | 0.0385 | >0.1 | 25% | 128 | Picked twice; once mixture with Vimentin, once HNRNPK | Protein and mRNA metabolism |
19 | −1.060 | >0.1 | 1.025 | >0.1 | >0.1 | 30% | 93 | - | |||
PCBP1 | Poly(rC) binding protein 1 | 57 | −1.375 | 0.0932 | −1.277 | 0.0018 | >0.1 | 43% | 96 | Mixture with ALDOC | Protein and mRNA metabolism |
PGK1 | Phosphoglycerate kinase 1 | 72 | −1.130 | >0.1 | −1.118 | 0.0299 | >0.1 | 60% | 176 | Confirmed twice | Energy metabolism |
73 | −1.513 | >0.1 | −1.117 | 0.0326 | >0.1 | 45% | 150 | Confirmed twice | |||
PRDX2 | Peroxiredoxin 2 | 78 | 1.742 | >0.1 | −1.088 | >0.1 | 0.0095 | 40% | 147 | Confirmed twice | Redox metabolism |
PSMA1 | Proteasome (prosome, macropain) subunit, alpha type, 1 | 51 | −1.189 | >0.1 | −1.059 | 0.0483 | 0.0487 | 58% | 132 | Confirmed twice | Protein and mRNA metabolism |
PSME2 | Proteasome (prosome, macropain) activator subunit 2 (PA28 beta) | 63 | 1.115 | >0.1 | −1.226 | 0.0120 | 0.0526 | 38% | 179 | Confirmed twice | Protein and mRNA metabolism |
TAGLN2 | Transgelin 2 | 67 | −1.026 | >0.1 | −1.190 | 0.0220 | >0.1 |  | 1Protb | - | NA |
TPI1 | Triosephosphate isomerase 1 | 7 | −1.350 | 0.0074 | −1.043 | >0.1 | >0.1 | NA | Spot mappingc | Energy and redox metabolism | |
11 | −1.430 | 0.0001 | −1.038 | >0.1 | >0.1 | 34% | 101 | - | |||
13 | −1.436 | 0.0043 | 1.015 | >0.1 | >0.1 | 95% | 192 | - | |||