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Table 4 Secondary antibodies used

From: Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cells

Secondary antibody

Application and dilution

Source

FITC conjugated goat anti rabbit IgG

IF 1:400

Jackson Immunoresearch, PA, USA, 111-095-144

Rhodamine Red-X conjugated goat anti mouse IgG

IF 1:400

Jackson Immunoresearch, PA, USA, 115-259-146

Dylight 649 conjugated goat anti chicken IgY

IF 1:400

Jackson Immunoresearch, PA, USA, 103-485-155

Dylight 649 conjugated donkey anti goat IgG

IF 1:400

Jackson Immunoresearch, PA, USA, 705-494-147

Alexa Fluor 594 conjugated donkey anti mouse IgG

IF 1:1,000

Life Technologies Inc, Ontario, Canada, 987237

Alexa Fluor 448 conjugated donkey anti rabbit IgG

IF 1:1,000

Life Technologies Inc, Ontario, Canada, 913921

Peroxidase-Affinipure Gt anti-mouse IgG

WB 1:7,500

Jackson ImmunoResearch, PA, USA, 115-035-174

Dot blot 1:7,500

  1. IF, Immunofluorescence; WB, Western blot.