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Table 4 Secondary antibodies used

From: Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cells

Secondary antibody Application and dilution Source
FITC conjugated goat anti rabbit IgG IF 1:400 Jackson Immunoresearch, PA, USA, 111-095-144
Rhodamine Red-X conjugated goat anti mouse IgG IF 1:400 Jackson Immunoresearch, PA, USA, 115-259-146
Dylight 649 conjugated goat anti chicken IgY IF 1:400 Jackson Immunoresearch, PA, USA, 103-485-155
Dylight 649 conjugated donkey anti goat IgG IF 1:400 Jackson Immunoresearch, PA, USA, 705-494-147
Alexa Fluor 594 conjugated donkey anti mouse IgG IF 1:1,000 Life Technologies Inc, Ontario, Canada, 987237
Alexa Fluor 448 conjugated donkey anti rabbit IgG IF 1:1,000 Life Technologies Inc, Ontario, Canada, 913921
Peroxidase-Affinipure Gt anti-mouse IgG WB 1:7,500 Jackson ImmunoResearch, PA, USA, 115-035-174
Dot blot 1:7,500
  1. IF, Immunofluorescence; WB, Western blot.