Table 3 Primary antibodies used
Primary antibody | Application and dilution | Description | Source |
|---|---|---|---|
MeCP2 (C-terminal) | IF 1:200 | Rabbit polyclonal | Millipore, Billerica, MA, USA, 07-013 |
MeCP2 (C-terminal) | WB 1:100 | Mouse monoclonal | Abcam, Ontario, Toronto, Canada, Ab50005 |
IF 1:200 | |||
MeCP2E1 | WB 1:100 | Chicken polyclonal | Custom-made [7] |
GFAP | IF 1:200 | Mouse monoclonal | Invitrogen, Life Technologies Inc, Burlington, Ontario, Canada 421262 |
TUBULIN III (TUB III) | IF 1:200 | Mouse monoclonal | Chemicon, Millipore, Billerica, MA, USA MAB1637 |
OLIG2 | IF 1:200 | Rabbit polyclonal | Millipore, Millipore, Billerica, MA, USA, AB9610 |
NESTIN | IF 1:230 | Rat monoclonal | Developmental Studies Hybridoma Bank, Rat-401c |
S100B | IF 1:100 | Mouse monoclonal | Abcam, Ontario, Toronto, Canada, ab4066 |
CNPase | IF 1:100 | Mouse monoclonal | Covance, SMI-91R |
MBP | IF 1:100 | Rabbit polyclonal | Abcam, Ontario, Toronto, Canada, ab40390 |
NEUN | IF 1:200 | Mouse monoclonal | Millipore, Billerica, MA, USA, Mab377 |
KI67 | IF 1:200 | Rabbit polyclonal | Santa cruz, Dallas, Texas, USA, sc-15402 |
5mC | Dot blot 1:1,000 | Mouse monoclonal | Abcam, Ontario, Toronto, Canada, Ab73938 |
IF 1:200 | |||
5hmC | Dot blot 1:1,000 | Rabbit polyclonal | Active Motif, 39769 |