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Table 3 Primary antibodies used

From: Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cells

Primary antibody Application and dilution Description Source
MeCP2 (C-terminal) IF 1:200 Rabbit polyclonal Millipore, Billerica, MA, USA, 07-013
MeCP2 (C-terminal) WB 1:100 Mouse monoclonal Abcam, Ontario, Toronto, Canada, Ab50005
IF 1:200
MeCP2E1 WB 1:100 Chicken polyclonal Custom-made [7]
GFAP IF 1:200 Mouse monoclonal Invitrogen, Life Technologies Inc, Burlington, Ontario, Canada 421262
TUBULIN III (TUB III) IF 1:200 Mouse monoclonal Chemicon, Millipore, Billerica, MA, USA MAB1637
OLIG2 IF 1:200 Rabbit polyclonal Millipore, Millipore, Billerica, MA, USA, AB9610
NESTIN IF 1:230 Rat monoclonal Developmental Studies Hybridoma Bank, Rat-401c
S100B IF 1:100 Mouse monoclonal Abcam, Ontario, Toronto, Canada, ab4066
CNPase IF 1:100 Mouse monoclonal Covance, SMI-91R
MBP IF 1:100 Rabbit polyclonal Abcam, Ontario, Toronto, Canada, ab40390
NEUN IF 1:200 Mouse monoclonal Millipore, Billerica, MA, USA, Mab377
KI67 IF 1:200 Rabbit polyclonal Santa cruz, Dallas, Texas, USA, sc-15402
5mC Dot blot 1:1,000 Mouse monoclonal Abcam, Ontario, Toronto, Canada, Ab73938
IF 1:200
5hmC Dot blot 1:1,000 Rabbit polyclonal Active Motif, 39769
  1. MeCP2, Methyl CpG binding protein; GFAP, Glial fibrillary acidic protein; OLIG2, Oligodendrocyte lineage transcription factor 2; CNPase, 2',3'-Cyclic-nucleotide 3'-phosphodiesterase, MBP, Myelin basic protein; NEUN, NEUronal Nuclei; 5mC, 5-methylcytosine; 5hmc, 5-hydroxymethylcytosine; IF, Immunofluorescence; WB, Western blot.