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Table 2 List of primers used for qRT-PCR

From: Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cells

Gene Direction Sequence Reference
Mecp2 (total) Forward 5′-GGTAAAACCCGTCCGGAAAATG-3′ [4]
Reverse 5′-TTCAGTGGCTTGTCTCTGAG-3′
Mecp2e1 Forward 5′-AGGAGAGACTGGAGGAAAAGT-3′ [5]
Reverse 5′-CTTAAACTTCAGTGGCTTGTCTCTG-3′
Mecp2e2 Forward 5′-CTCACCAGTTCCTGCTTTGATGT-3′
Reverse 5′-CTTAAACTTCAGTGGCTTGTCTCTG-3′
Tubulin III (Tub III ) Forward 5′-TCAGCGATGAGCACGGCATA-3′ [37]
Reverse 5′-CACTCTTTCCGCACGACATC-3′
Gfap Forward 5′-GCTCACAATACAAGTTGTCC-3′
Reverse 5′-ACCTAATTACACAGAGCCAGG-3′
Gapdh Forward 5′-AACGACCCCTTCATTGAC-3′ [43]
Reverse 5′-TCCACGACATACTCAGCAC-3′
NeuN Forward 5′-GGCAATGGTGGGACTCAAAA-3′ [44]
Reverse 5′-GGGACCCGCTCCTTCAAC-3′
S100b Forward 5′-GCTGACCACCATGCCCCTGTAG-3′ [45]
Reverse 5′-CTGGCCATTCCCTCCTCTGTC-3′
Mbp Forward 5′-GGCACGCTTTCCAAAATCT-3′ [46]
Reverse 5′-CCATGGGAGATCCAGAGC-3′
Cnpase Forward 5′-CATCCTCAGGAGCAAAGGAG-3′ [47]
Reverse 5′-TGAATAGCGTCTTGCACTCG-3′
Dnmt1 Forward 5′-AGGGAAAAGGGAAGGGCAAG-3′ [48]
Reverse 5′-AGAAAACACATCCAGGGTCCG-3′
Dnmt3a Forward 5′-CAGCGTCACACAGAAGCATATCC-3′
Reverse 5′-GGTCCTCACTTTGCTGAACTTGG-3′
Dnmt3b Forward 5′-CCTGCTGAATTACTCACGCCCC-3′
Reverse 5′-GTCTGTGTAGTGCACAGGAAAA-3′
Xist Forward 5′-TTGTGGCTTGCTAATAAT-3′ [39]
Reverse 5′-AAACCCCATCCTTTATG-3′