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Figure 6 | Molecular Autism

Figure 6

From: Decitabine alters the expression of Mecp2 isoforms via dynamic DNA methylation at the Mecp2 regulatory elements in neural stem cells

Figure 6

Bisulfite pyrosequencing analysis of DNA methylation at the Methyl CpG binding protein 2 gene ( Mecp2 ) regulatory elements after decitabine treatment. (A) Effect of decitabine exposure at D2 on the percentage DNA methylation of Mecp2 regulatory regions. The three promoter regions are (a) R1, (b) R2, (c) R3, and the three intron 1 regions are (d) R4, (e) R5, and (f) R6. (B) Effect of decitabine exposure on average methylation over the entire region 2 (R2) (a), and intron 1 (R4 to R6) (b). Significant differences from controls: **P <0.01; *P <0.05; n = 3 ± standard error of the mean. (C) Effect of decitabine withdrawal at D8 on percentage methylation of Mecp2 regulatory regions. The regions are promoter regions (a) R1, (b) R2 and (c) R3, and intron 1 regions (d) R4, (e) R5 and (f) R6. (D) Effect of decitabine withdrawal on average DNA methylation over entire region 2 (R2) (a), and intron 1 (R4 to R6) (b).

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