Methyl CpG binding protein 2 gene ( Mecp2 ) isoform-specific transcript expression and DNA methylation at the Mecp2 regulatory elements during neural stem cell (NSC) differentiation. (A) Analysis of Mecp2e1 and Mecp2e2 transcript levels during NSC differentiation: n = 3 ± standard error of the mean (SEM). Significant differences: ***P <0.001; **P <0.01; *P <0.05. (B) Average percentage methylation over Mecp2 promoter and intron 1 regions at day 0 (D0), day 2 (D2) and day 8 (D8) during NSC differentiation. The regions are promoter regions R1, CpG island contains 13 CpG sites; R2, 4 CpG sites; R3, 2 CpG sites, and intron 1 regions R4, 1 CpG site; R5, 1 CpG site; and R6, 2 CpG sites; n = 3 ± SEM. Significant differences: **P <0.01; *P <0.05. Gapdh, glyceraldehyde-3-phosphate dehydrogenase gene.